ANALYSIS OF ENCODING GENE FOR PALMITIC ACID CONTENT OF WINGED BEAN (Pshopocarpus tetragonolobus (L.) DC) SEEDS

Volume 6, Issue 6, December 2021     |     PP. 115-128      |     PDF (394 K)    |     Pub. Date: December 28, 2021
DOI: 10.54647/biology18197    77 Downloads     5586 Views  

Author(s)

Nurtjahjo Dwi Sasongko, Faculty of Biology The University of Jenderal Soedirman, Purwokerto-Indonesia
Adi Amurwanto, Faculty of Biology The University of Jenderal Soedirman, Purwokerto-Indonesia

Abstract
Winged bean (Psophocarpus tetragonolobus (L.) (DC.) contains moderate to high fat in its seeds including palmitic acid (C18:0). Of those three sampling areas in Indonesia, the Sumateranese winged bean seeds contains 60%; which is important raw source of various oleochemical industries. Palmitoyl ACP is one among those enzyme involves in palmitic acid biosynthesis pathway from fatty acid synthase. Information about encoding genes of ACP enzyme which involves in palmitic acid biosynthesis pathway, therefore, becomes a prerequisite and became the aim of this study. Current study designed three different specific primer for palmitic acids namely: A. palmitoyl-ACP, B. KAS III, and C. acc D. each of them has the following nucleotide sequences: A. forward primer (F) 5’-GTC GTG CAT CGG GGA AGA A -3’ and reverse (R) 5’-TCG CTC CAT TTA GGC TGC AA-3’. B. KAS III (F) 5’-TGC CAG ACA CCA TCA CAA ACT-3’ and (R) 5’-TGA CGC CAG CGA TTA CAG C-3’ and C. acc D (F) 5’-GGG CGT GAT GGA GTT TG-3’ and (R) 5’-AGG TTG GCC TCG TTC TG-3’ which were tested on three different genotypes the short pod winged beans, medium length pods and long pods ones on a PCR machine. The PCR resulted in amplicons of 400 bp from two genotypes only (short and medium length pods genotypes) but nit to long pod genotype. Interestingly that the fragments appeared at the same locus though they were characterized as different genotypes, this locus was then predicted as putative fragments of the palmitoyl ACP enzyme encoding gene in winged bean seed involves in the palmitic acid biosynthesis.

Keywords
palmitic acid, the palmitoyl ACP enzyme, primers design, winged beans

Cite this paper
Nurtjahjo Dwi Sasongko, Adi Amurwanto, ANALYSIS OF ENCODING GENE FOR PALMITIC ACID CONTENT OF WINGED BEAN (Pshopocarpus tetragonolobus (L.) DC) SEEDS , SCIREA Journal of Biology. Volume 6, Issue 6, December 2021 | PP. 115-128. 10.54647/biology18197

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