In vitro Pneumovirus and Paramixovirus infection is modulated by the passage of mesenchymal stem cells

Karla Zarate, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), CP 04510, Mexico City, Mexico

Xóchitl Ambriz, BioLabs NTX 3033 Irving Blvd, Dallas TX 75247, USA

Javier R. Ambrosio, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), CP 04510, Mexico City, Mexico

Rocio Tirado, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), CP 04510, Mexico City, Mexico

Abstract
The human respiratory syncytial virus (hRSV), the human metapneumovirus (hMPV), and the human parainfluenza virus (hPIV) are the main etiological agents of acute respiratory infections in children and adults. Human mesenchymal stem cells (hMSCs) are also infected by these viruses. These cells differentially express receptors according to the number of passages, thereby, influencing susceptibility and permissiveness to viral infections. The aim of this study was to determine the susceptibility of amniotic hMSC to hRSV, hMPV, and hPIV. We examined the effect of hMSC passages on the viral gene expression by endpoint RT-PCR and the viral production by TCID50 and evaluated the effect on the hMSC cytoskeleton by light microscopy. We found that the viral titer increased with respect to the number of hMSC passages. This coincided with the highest gene expression levels documented at the same passages. As for the hMSC morphological changes, we suggest that these changes were associated with actin modifications. Taken together, viral infections of hMSCs cause altered gene expression and cytoskeleton morphology, with the viral loads ascending as a function of the number of passages.

Keywords
Mesenchymal stem cells; human respiratory viral infection; hRSV; hMPV; hPIV; cytoskeleton; susceptibility; permissiveness.

Cite this paper
Karla Zarate, Xóchitl Ambriz, Javier R. Ambrosio, Rocio Tirado, In vitro Pneumovirus and Paramixovirus infection is modulated by the passage of mesenchymal stem cells , SCIREA Journal of Clinical Medicine. Volume 7, Issue 3, June 2022 | PP. 167-179. 10.54647/cm32819

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