Fingerprint identification of p. notoginseng flower, p. ginseng flower and p. quinquefolium lower based by HPLC and NIRS

Author(s)

Abstract
Abstract: Aims: In view of the current study on the fingerprint of panax pseudo-ginseng, panax ginseng and panax quinquefolium, the identification features can not be clearly distinguished. HPLC and NIRS analysis technologies were used to establish the fingerprint of p. notoginseng flower, p. ginseng flower and p. quinquefolium flower, and to compare and analyze the differences among them. Methods: By comparing the retention time of reference substances peaks, identified peaks, established fingerprints, and analyzed and compared the different characteristics of fingerprint of each variety. The WinISI II NIRS analysis software was used to establish the original NIRS fingerprint map of each variety, the first-order and the second-order derivative processing fingerprint map, to analyze and compare the different characteristics of the fingerprint map of each variety. Results: Six peaks of p. notoginseng flower Re, R1, Rb1, Rc, Rb3 and Rd were identified, five peaks of p. ginseng flower Re, R1, Rb1, Rc and Rd were identified, and five peaks of panax quinquefolium Re, Rb1, Rc, Rb3 and Rd were identified. p. notoginseng flower (S) and p. ginseng flower (R) features of HPLC fingerprint differences mainly in peak S16, S20, S22, S23, S30, S31, S32. HPLC fingerprint characteristics of p. notoginseng flower (S) and p. quinquefolius flower (X) were mainly in peak S16, X13, X14, X23, X24, X25, X35 X36, X40. HPLC fingerprint features of p. ginseng flowers and p. quinquefolius were mainly characterized by peak R27, X20, X21, X34, X36, X37, X38. The absorption peaks of p. notoginseng flower NIRS original fingerprint at 2000~2200 nm were different from others. The first-derivative fingerprint of p. notoginseng flower and p. quinquefolium flower had peaks between 2300~2350 nm, while the p. ginseng flower were not.The peaks of p. ginseng flowers and American ginseng flowers between 2090~2150 nm, p. notoginseng flowers were without. The peaks between 1670~1690 nm of the fingerprint of p. notoginseng flower were different from others. The second derivative of p. notoginseng at 2250~2300 nm were different from others, and there were not difference at 2300~2350 nm between p. notoginseng flower and p. quinquefolium flower, while different from p. ginseng flowers. Conclusion: HPLC and NIRS fingerprints of the flower of p. notoginseng, p. ginseng and p. quinquefolium could be used to distinguish and identify them. HPLC fingerprint were identified remarkable and NIRS fingerprint could quickly identify them with zero damage.The fingerprint identification method established in this paper had the advantages of obvious features and shortened analysis time.It provided a reliable reference for further identification and research and development of p. notoginseng flower, p. ginseng flower and p. quinquefolium flower.

Keywords
Keywords: p. notoginseng flower; p. ginseng flower; p. quinquefolium flower; HPLC fingerprint; NIRS fingerprint

Cite this paper
Huang Z.Q., Gao M.J., Feng G.Q, Ma X.S, Wang R, Fingerprint identification of p. notoginseng flower, p. ginseng flower and p. quinquefolium lower based by HPLC and NIRS , SCIREA Journal of Clinical Medicine. Volume 4, Issue 1, February 2019 | PP. 11-30.

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